Development and validation of a liquid chromatography-tandem mass spectrometry method for the determination of nicotine and its metabolites in placenta and umbilical cord

作者：Paniagua Gonzalez Lucia; Jimenez Morigosa Cristian; Lendoiro Elena; Concheiro Marta; Cruz Angelines; Lopez Rivadulla Manuel; de Castro Ana^*

来源：Drug Testing and Analysis, 2018, 10(8): 1305-1314.

DOI：10.1002/dta.2381

• LC-MS
• MS
• nicotine
• placenta
• tobacco
• umbilical cord

Tobacco exposure during pregnancy is associated with obstetric and fetal complications. We developed and validated a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to determine nicotine, cotinine, and hydroxycotinine (OH-cotinine) in placenta (PL) and umbilical cord (UC). Specimens were homogenized in water, followed by solid-phase extraction. Chromatographic separation was performed using an Atlantis (R) HILIC Silica column. Detection was accomplished in electrospray in positive mode. Method validation included: linearity (5 to 1000ng/g), accuracy (86.9 to 105.2% of target concentration in PL, and 89.1 to 105.0% in UC), imprecision (6.8 to 11.8% in PL, and 7.6 to 12.2% in UC), limits of detection (2ng/g for cotinine and OH-cotinine, and 5ng/g for nicotine) and quantification (5ng/g), selectivity (no endogenous or exogenous interferences), matrix effect (-34.1 to -84.5% in PL, %CV=9.1-24.0%; -18.9 to -84.7% in UC, %CV=10.2-23.9%), extraction efficiency (60.7 to 131.5% in PL, and 64.1 to 134.2% in UC), and stability 72h in the autosampler (<11.5% loss in PL, and<13% loss in UC). The method was applied to 14 PL and UC specimens from tobacco users during pregnancy. Cotinine (6.8-312.2ng/g in PL; 6.7-342.3ng/g in UC) was the predominant analyte, followed by OH-cotinine (

• 出版日期2018-8
• 单位司法部司法鉴定科学技术研究所