Differential responses of GC-1 spermatogonia cells to high and low doses of bisphenol A

作者：Li, Yuhua; Duan, Fei; Zhou, Xiaoyu; Pan, Hongjie; Li, Runsheng^*

来源：Molecular Medicine Reports, 2018, 18(3): 3034-3040.

DOI：10.3892/mmr.2018.9256

• bisphenol A
• spermatogonia
• GC-1
• methylation

Bisphenol A (BPA) is an environmental endocrine disruptor. The exact effect of BPA on spermatogenesis and the specific epigenetic effects on mouse spermatogonia remain to be elucidated. The present study exposed the GC-1 spermatogonial cell line to a series of differing BPA concentrations and examined the subsequent effects on cell proliferation, mitogen activated protein kinase (MAPK) signaling, DNA and histone methylation. A Cell Counting Kit-8 assay revealed that BPA significantly inhibited cell growth at the concentration of 10 mu g/ml, however no significant alterations were detected at lower BPA doses. The global DNA methylation levels were reduced at the dose of 10 mu g/ml of BPA, via detection of 5-methylcytosine using a dot blot. The protein and mRNA expression levels of DNA methyltransferase (DNMT) 1 were decreased at 10 and mu g/ml of BPA, detected via western blotting and reverse transcription-quantitative polymerase chain reaction, respectively. The global levels of H3K27me3 was decreased at 10 mu g/ml BPA, detected via western blotting. Increased phosphorylation of p38 and decreased phosphorylation of extracellular signal-regulated kinases 1/2 were observed at 10 and 1 mu g/ml BPA. The results demonstrated that high and low doses of BPA exposure exhibit differential effects on cell growth, global DNA methylation, histone H3K9Me3 and H3K27Me3 levels and additionally affect the MAPK signaling pathways.

• 出版日期2018-9
• 单位上海市计划生育科学研究所; 复旦大学