Active tumor-therapeutic liposomal bacteriobot combining a drug (paclitaxel)-encapsulated liposome with targeting bacteria (Salmonella Typhimurium)

作者：Van Du Nguyen; Han Ji Won; Choi Young Jin; Cho Sunghoon; Zheng Shaohui; Ko Seong Young; Park Jong Oh^*; Park Sukho

来源：Sensors and Actuators B: Chemical , 2016, 224: 217-224.

DOI：10.1016/j.snb.2015.09.034

• Liposome
• Microrobot
• Salmonella Typhimurium
• Paclitaxel
• Tumor therapy
• Bacterial actuation

We propose a new tumor-therapeutic bacteria-based microrobot (bacteriobot) combining a paclitaxel-loaded liposomal microcargo with tumor-targeting Salmonella Typhimurium bacteria. The tumor-therapeutic liposomal bacteriobot was constructed by binding biotin molecules displayed on the outer membrane proteins of the bacteria and streptavidin coated on the drug-loaded liposomes. First, we performed a motility analysis of the bacteriobot, where the bacteria-actuated liposomes showed much higher average velocity (3.09 +/- 0.44 mu m/s) than the liposomes without bacterial actuation (0.40 +/- 0.14 mu m/s). Second, we performed a cytotoxicity test using a breast cancer cell line (4T1) to check the tumor-therapeutic efficacy of the bacteriobots. The drug-loaded bacteriobots (IC50 = 16.48 + 0.43 mu g/ml) showed better tumor-killing ability than the drug-containing liposomes (IC50 = 21.91 +/- 0.74 mu g/ml). Moreover, the bacteriobots showed strong tumor-targeting and killing properties in a simple co-culture chamber containing normal cells (NIH/3T3) and cancer cells (4T1). These results revealed that the constructed bacteriobots can be used for active tumor therapy.

• 出版日期2016-3-1