A 3D Osteoblast In Vitro Model for the Evaluation of Biomedical Materials

作者:Restle Luciana; Costa Silva Daniela; Lourenco Emanuelle Stellet; Bachinski Rober Freitas; Batista Ana Carolina; Ribeiro Linhares Adriana Brandao; Alves Gutemberg Gomes
来源:Advances in Materials Science and Engineering, 2015, 2015: 268930.
DOI:10.1155/2015/268930

摘要

<jats:p>Biomedical materials for bone therapy are usually assessed for their biocompatibility and safety employing animal models or<jats:italic>in vitro</jats:italic>monolayer cell culture assays. However, alternative<jats:italic>in vitro</jats:italic>models may offer controlled conditions closer to physiological responses and reduce animal testing. In this work, we developed a 3D spheroidal cell culture with potential to evaluate simultaneously material-cell and cell-cell interactions. Different cell densities of murine MC3T3-E1 preosteoblasts or human primary osteoblasts (HOb) were used to determine the ideal procedure of spheroidal cultures and their adequacy to material testing. Cells were seeded on 96-well plates coated with agar and incubated in agitation from 1 to 7 days. Aggregate morphology was qualitatively evaluated considering the shape, size, repeatability, handling, and stability of spheroids. Higher cell densities induced more stable spheroids, and handling was considered appropriate starting from 2 × 10<jats:sup>4</jats:sup>cells. Confocal microscopy and Scanning Electron Microscopy indicate that most cells within the aggregate core are viable. Exposure to positive controls has shown a dose dependent cell death as measured by XTT assay. Aggregates were stable and presented good viability when employed on standardized testing of metallic and polymer-based biomaterials. Therefore, osteoblast spheroids may provide a promising tool for material screening and biocompatibility testing.</jats:p>

  • 出版日期2015

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