A selectivity filter at the intracellular end of the acid-sensing ion channel pore

作者：Lynagh Timothy; Flood Emelie; Boiteux Celine; Wulf Matthias; Komnatnyy Vitaly V; Colding Janne M; Allen Toby W; Pless Stephan A^*

来源：eLife, 2017, 6: e24630.

DOI：10.7554/eLife.24630

Increased extracellular proton concentrations during neurotransmission are converted to excitatory sodium influx by acid-sensing ion channels (ASICs). 10-fold sodium/potassium selectivity in ASICs has long been attributed to a central constriction in the channel pore, but experimental verification is lacking due to the sensitivity of this structure to conventional manipulations. Here, we explored the basis for ion selectivity by incorporating unnatural amino acids into the channel, engineering channel stoichiometry and performing free energy simulations. We observed no preference for sodium at the "GAS belt" in the central constriction. Instead, we identified a band of glutamate and aspartate side chains at the lower end of the pore that enables preferential sodium conduction.

• 出版日期2017-5-12