Assessment of the bacterial diversity in soils: Evolution of approaches and methods

作者：Dobrovol'skaya T G^*; Golovchenko A V; Pankratov T A; Lysak L V; Zvyagintsev D G

来源：Eurasian Soil Science, 2009, 42(10): 1138-1147.

DOI：10.1134/S1064229309100081

This review analyzes the publications of Russian and foreign microbiologists presenting new approaches and methods for assessing the bacterial diversity of soils in the last twenty years. Using the example of peat soils, it is shown how the concepts of the diversity of the bacterial communities changed in conformity with the evolution of the analytical methods-from the traditional cultural to the molecular-biological ones. The data on the new phylotypes, genera, and species of bacteria adapted to growth in the acid medium and low temperatures characteristic of bog ecosystems are presented. Presently, one of the principal problems of soil microbiology is the necessity of the transfer from the databases on the microbial diversity constructed on the basis of molecular-biological methods to the analysis of the ecological functions of soil microorganisms. The prospects of the ecological evaluation of the bacterial diversity in soils based on the integration of different methods are discussed.

• 出版日期2009-10