A Multiplex PCR-Based Molecular Identification of Five Morphologically Related, Medically Important Subgenus Stegomyia Mosquitoes from the Genus Aedes (Diptera: Culicidae) Found in the Ryukyu Archipelago, Japan

作者：Higa Yukiko^*; Toma Takako; Tsuda Yoshio; Miyagi Ichiro

来源：Japanese Journal of Infectious Diseases, 2010, 63(5): 312-316.

Internal transcribed spacer regions of ribosomal DNA were sequenced, and new species-specific primers were designed to simplify the molecular identification of five morphologically related subgenus Stegomyia mosquito species Aedes aegypti, Ae. albopictus, Ae. riversi, Ae. flavopictus, and Ae. daitensis found in the Ryukyu Archipelago, Japan. Each newly designed primer was able to amplify a species-specific fragment with a different size. Conditions for multiplex PCR were optimized to identify all five species in a single PCR. This method is a convenient tool for entomological field surveys, particularly in arbovirus endemic/epidemic areas where some of these species coexist.

• 出版日期2010-9