Direct binding of Concanvalin A onto iron oxide nanoparticles for fast magnetic selective separation of lactoferrin

作者：Lai Bo Hung; Chang Chih Hao; Yeh Chun Chieh; Chen Dong Hwang^*

来源：Separation and Purification Technology, 2013, 108: 83-88.

DOI：10.1016/j.seppur.2013.02.020

• Concanavalin A
• Iron oxide nanoparticles
• Glycoprotein
• Lactoferrin
• Magnetic separation

Concanavalin A (Con A) was successfully bound onto Fe3O4 magnetic nanoparticles (MNPs) via carbodiimide activation as a magnetic nano-adsorbent for glycoprotein separation. The resulting Con A-bound Fe3O4 MNPs were nearly superparamagnetic with a mean diameter of 11.74 +/- 3.86 nm. The size and structure of Fe3O4 MNPs had no significant changes after Con A binding. The binding of Con A on the surface of Fe3O4 MNPs was confirmed by the analysis of Fourier transform infrared spectroscopy. From the thermogravimetric analysis and the concentration change of Con A in the binding solution, the weight percentage of Con A in the typical Con A-bound Fe3O4 MNPs was determined to be about 10 wt%. Furthermore, the Con A-bound Fe3O4 MNPs were found to be quite efficient for the fast adsorption of lactoferrin. The equilibrium was achieved within 5 min and the adsorption data for lactoferrin obeyed the Langmuir isotherm equation. At pH 7 and 25 degrees C, the maximum adsorption capacity and equilibrium constant were 59.2 mg/g and 0.0103 L/mg, respectively. In addition, by SDS-polyacrylamide gel electrophoresis, the capability of Con A-bound Fe3O4 MNPs for the selective adsorption of lactoferrin was further confirmed.

• 出版日期2013-4-19