Well-Defined Glycopolymers via RAFT Polymerization: Stabilization of Gold Nanoparticles

作者：Alvarez Paino Marta; Bordege Vanesa; Cuervo Rodriguez Rocio; Munoz Bonilla Alexandra^*; Fernandez Garcia Marta

来源：Macromolecular Chemistry and Physics, 2014, 215(19): 1915-1924.

DOI：10.1002/macp.201400306

• glycopolymers
• gold nanoparticles
• lectins
• molecular recognition
• RAFT polymerization

Well-defined glycopolymers of poly(2-{[(d-glucosamin-2-N-yl)carbonyl]oxy}ethyl meth(acrylate)) (PHEMAGl and PHEAGl, respectively) are first synthesized by reversible addition-fragmentation chain transfer (RAFT) polymerization in green solvents such as water and dimethyl sulfoxide (DMSO). The biomolecular recognition of the synthesized glycopolymers bearing glucose residues toward Concanavalin A lectin is confirmed by turbidimetric assays. Subsequently, HEMAGl glycopolymer is mixed with gold nanoparticles in the presence of sodium borohydride, which reduces the terminal groups to thiol and yields the preparation of synthetic carbohydrate polymer-stabilized gold nanoparticles showing enhanced colloidal stability. The resulting glyco-nanoparticles are characterized by dynamic light scattering (DLS) and field-emission electron microscopy (FE-SEM), whereas the molecular recognition abilities are investigated using UV-vis spectroscopy by analyzing the shift in the plasmon band as a consequence of the aggregation variation with the addition of Concanavalin A.

• 出版日期2014-10