Anti-inflammatory effects of Terminalia phanerophlebia (Combretaceae) and identification of the active constituent principles

作者：Nair J J; Aremu A O; Van Staden J^*

来源：South African Journal of Botany, 2012, 81: 79-80.

DOI：10.1016/j.sajb.2012.06.001

• Anti-inflammatory
• Stigmast-4-ene-3,6-dione
• Terminalia phanerophlebia
• beta-Sitostenone
• beta-Sitosterol

The %26apos;Lebombo cluster leaf%26apos; Terminalia phanerophlebia (Combretaceae) is used in traditional medicine in South Africa for a host of ailments, including aches and pain, wounds and infections, gynaecological conditions, inflammation, mental illness such as epilepsy, and sexually-transmitted diseases. Based on the anti-inflammatory activity (92.4% inhibition of the cyclooxygenase enzyme COX-2) initially observed for a crude extract, stems of T phanerophlebia were here examined for the underlying phytochemical principles. Three cholestane tritemenoids beta-sitosterol, beta-sitostenone and stigmast-4-ene-3,6-dione were isolated from the ethanol extract by silica gel column chromatography via gradient elution with hexane/ethyl acetate mixtures. Structure elucidation was facilitated by use of physical and spectroscopic methods, including high field 2D NMR and mass spectroscopic techniques. The crude extract as well as beta-sitosterol was seen to be selective inhibitors of the cyclooxygenase enzyme COX-2. This work is of chemosystematic relevance as it represents the first isolation of Is-sitostenone and stigmast-4-ene-3,6-dione from any species of Tenninalia.

• 出版日期2012-7