An improved method for the simultaneous determination of photosynthetic O-2 evolution and CO2 consumption in Rhizophora mucronata leaves

作者：Ulqodry T Z; Nose A^*; Zheng S H

来源：Photosynthetica, 2016, 54(1): 152-157.

DOI：10.1007/s11099-015-0166-6

• carbon dioxide consumption
• oxygen evolution
• photosynthetic performance

The photosynthetic gas-exchange has been assessed traditionally either as O-2 evolution or CO2 consumption. In this study, we used a liquid-phase O-2 electrode combined with CO2 optodes to examine simultaneously photosynthesis in intact leaves of mangrove Rhizophora mucronata. We verified suitable conditions for leaf photosynthetic rates by assessing pH levels and NaHCO3 concentrations and compared these to the gas-exchange method at various PAR levels. The photosynthetic rate in response to pH exhibited a similar pattern both for O-2 evolution and CO2 consumption, and higher rates were associated with intermediate pH compared with low and high pH values. The net photosynthetic quotient (PQ) of R. mucronata leaves ranged from 1.04-1.28. The PQ values, which were never lesser than 1, suggested that photorespiration did not occur in R. mucronata leaves under aqueous conditions. The similar maximum photosynthetic rates suggested that all measurements had a high capacity to adjust the photosynthetic apparatus under a light saturation condition. The simultaneous measurements of O-2 evolution and CO2 consumption using the Clark oxygen electrode polarographic sensor with the CO2 optode sensor provided a simple, stable, and precise measurement of PQ under aqueous and saturated light conditions.

• 出版日期2016-3