Wavelength encoding technique for particle analyses in hematology analyzer

作者:Rongeat Nelly*; Brunel Patrick; Gineys Jean Philippe; Cremien Didier; Couderc Vincent; Nerin Philippe
来源:Optics Express, 2011, 19(15): 14076-14082.
DOI:10.1364/OE.19.014076

摘要

The aim of this study is to combine multiple excitation wavelengths in order to improve accuracy of fluorescence characterization of labeled cells. The experimental demonstration is realized with a hematology analyzer based on flow cytometry and a CW laser source emitting two visible wavelengths. A given optical encoding associated to each wavelength allows fluorescence identification coming from specific fluorochromes and avoiding the use of noisy compensation method.

  • 出版日期2011-7-18