Association Between Polymorphisms of ERCC1 and XPD and Clinical Response to Platinum-Based Chemotherapy in Advanced Non-Small Cell Lung Cancer

作者：Li, Fan; Sun, Xinchen^*; Sun, Ning; Qin, Shukui; Cheng, Hongyan; Feng, Jifeng; Chen, Baoan; Cheng, Lu; Lu, Zuhong; Ji, Jiazhong; Zhou, Yingfeng

来源：American Journal of Clinical Oncology: Cancer Clinical Trials , 2010, 33(5): 489-494.

DOI：10.1097/COC.0b013e3181b9cedc

• single nucleotide polymorphism
• gene-chip
• ERCC1
• XPD
• nucleotide excision repair
• NSCLC
• chemotherapy

Background and Objective: DNA repair capacity is correlated with sensitivity of cancer cells toward platinum-based chemotherapy. The aim of this study was to investigate whether single nucleotide polymorphisms (SNPs) in polymorphisms of DNA repair gene ERCC1 (excision repair cross-complementation group 1) and XPD (ERCC2, excision repair cross-complementation group 2) were associated with the tumor response in advanced non-small- cell lung cancer (NSCLC) patients received platinum-based chemotherapy in Chinese population.
Methods: Totally 115 patients with advanced NSCLC were routinely treated with cisplatin-or carboplatin-based chemotherapy, and clinical response was evaluated after 2 cycles. Three dimensions (3-D) polyacrylamide gel-based DNA microarray method was used to evaluate the genotypes of ERCC1 Asn118Asn (354 CT), Gln504Lys (8092 CA) and XPD Lys751Gln (35931 AC).
Results: The C -> T change of ERCC1 Asn118Asn polymorphism and the C -> A change of ERCC1 Gln504Lys polymorphism have statistically significant association with elevated or descendent platinum-based chemotherapy response respectively.
Conclusion: The polymorphic status of ERCC1 might be the promising ancillary marker for predicting treatment response of advanced stage NSCLC patients. The DNA microarray-based method is accurate, high-throughput and inexpensive, suitable for SNP genotyping in a large number of individuals.

• 出版日期2010-10
• 单位中国人民解放军第八一医院; 东南大学