Modification of fluorous substrates with oligo(ethylene glycol) via "click" chemistry for long-term resistance of cell adhesion

作者：Contreras Caceres Rafael^*; Santos Catherine M; Li Siheng; Kumar Amit; Zhu Zhiling; Kolar Satya S; Casado Rodriguez Miguel A; Huang Yongkai; McDermott Alison; Manuel Lopez Romero Juan; Cai Chengzhi

来源：Journal of Colloid and Interface Science, 2015, 458: 112-118.

DOI：10.1016/j.jcis.2015.07.033

• Click reaction
• Fluorinated substrate
• Fluorine-fluorine interaction
• OEG
• Protein resistance
• Cell adhesion resistance

In this work perfluorinated substrates fabricated from SiO2 glass slides are modified with oligo(ethylene glycol) (OEG) units for long-term resistance of cell adhesion purposes, based on fluorous interactions and click chemistry. Specifically, fluorous substrates, prepared by treatment of glass slides with 1 H, 1H, 2H, 2H-perfluorodecyltrimethoxysilane (FAS17), were coated with ethynyl-OEG-C8F17, followed by covalent attachment of an azido-OEG via copper-catalyzed azide-alkyne cycloaddition (CuAAC) "click" reaction. We demonstrate that the resultant surface avoid fibrinogen adsorption and resisted cell adhesion for over 14 days. X-ray photoemission spectroscopy (XPS) analysis and contact angle goniometry measurements confirm the presence of the OEG molecules on the fluorous substrates. Bright field optical images show total absence of 313 fibroblast cells on the OEG modified fluorinated substrate for 1 and 5 days, and a remarkably decrease of cell adhesion at 14 days.

• 出版日期2015-11-15