A highly selective near-infrared fluorescent probe for imaging H2Se in living cells and in vivo

作者：Kong, Fanpeng; Ge, Lihong; Pan, Xiaohong; Xu, Kehua^*; Liu, Xiaojun; Tang, Bo^*

来源：Chemical Science, 2016, 7(2): 1051-1056.

DOI：10.1039/c5sc03471j

• THIOREDOXIN REDUCTASE
• NITRIC-OXIDE
• TURN-ON
• SELENIUM SUPPLEMENTATION
• CANCER PREVENTION
• METABOLISM
• OXYGEN
• TRIAL
• FLUOROPHORES
• CONSTRUCTION

Hydrogen selenide (H2Se), a highly reactive Se species, is an important selenium metabolism intermediate involved in many physiological and pathological processes. This compound is of scientific interest with regard to the real-time monitoring of H2Se in living cells and in vivo to understand the anti-cancer mechanism of selenium. However, monitoring H2Se in living cells is still challenging due to the lack of straight forward, highly selective and rapid methods. Here, we developed a novel small-molecule fluorescent probe, NIR-H2Se, for imaging endogenous H2Se. NIR-H2Se exhibited high selectivity toward H2Se over selenocysteine (Sec), H2S and small molecule thiols and was successfully used to image the H2Se content in HepG(2) cells during Na2SeO3-induced apoptosis. Increased H2Se content and reduced ROS levels were observed under hypoxic conditions compared to normoxic conditions, which indicated that the cell apoptosis induced by Na2SeO3 under a hypoxic environment is via a non-oxidative stress mechanism. Thus, this probe should serve as a powerful tool for exploring the physiological function of H2Se and Se anticancer mechanisms in a variety of physiological and pathological contexts.

• 出版日期2016
• 单位山东师范大学