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摘要
The fluorescence labeling apovincaminic acid (AVA) and tracers apovincaminic acid-sulfadimethoxine were synthesized and used in the fluorescence polarization immunoassay method (FPIA) for the analysis of sulfadimethoxine (SMX). By using AVA as indicating materials, tracers apovincaminic acid-sulfadimethoxine (AVA-SMX) were prepared, which were purified by thin layer chromatography and identified by FPIA. The fluorescence properties of AVA and the characteristic performance of tracers immunoreacting with SMX antibodies were investigated by using tracers 5-aminofluorescein-sulfadimethoxine conjugates (AF-SMX) as a reference. The results indicated that the detection limit for AF-SMX was 2. 7 mu g/L, and the dynamic range for AVA-SMX was 0.5 - 146 mu g/L with a detection limit of 0.5 mu g/L. The sensitivity of the novel tracers was also estimated by dilution curves and calibration curves, respectively. The RSD of AVA-based FPIA method for SMX determination is 3.0%, and a good selectivity can be obtained with the solutions in the presence of the other sulfa drugs. The proposed AVA-based FPIA was used to determine SMX in feed and the result is well agreed with that obtained with HPLC.
