Aberrantly Expressed Genes and miRNAs in Slow Transit Constipation Based on RNA-Seq Analysis

作者：Zhao, Shipeng^*; Chen, Qiang^*; Kang, Xianwu; Kong, Bin; Wang, Zhuo

来源：Biomed Research International, 2018, 2018: 2617432.

DOI：10.1155/2018/2617432

• IRRITABLE-BOWEL-SYNDROME
• CHRONIC IDIOPATHIC CONSTIPATION
• COLORECTAL-CANCER
• COLON-CANCER
• ULCERATIVE-COLITIS
• PHARMACOLOGICAL MANAGEMENT
• PROGNOSTIC VALUE
• GUT MICROBIOTA
• UNITED-STATES
• DISEASE

Background. This study aims to identify the key genes and miRNAs in slow transit constipation (STC). Methods. M RNA and miRNA expression profiling were obtained. Differentially expressed genes (DEGs) and miRNAs were identified followed by the regulatory network construction. Functional annotation analysis and protein-protein interaction (PPI) network were conducted. The electronic validation was performed. Results. Hsa-miR-2116-3p, hsa-miR-3622a-5p, hsa-miR-424-5p, and hsa-miR-1273-3p covered most DEGs. HLA-DRB1, HLA-DRB5, C3, and ICAM were significantly involved in staphylococcus aureus infection. The PPI network generated several hub proteins including ZBTB16, FBN1, CCNF, and CDK1. Electronic validation of HLA-DRB1, PTGDR, MKI67, BIRC5, CCNF, and CDK1 was consistent with the RNA-sequencing analysis. Conclusion. Our study might be helpful in understanding the pathology of STC at the molecular level.

• 出版日期2018
• 单位河北医科大学