A new antibiotic produced by the cyanobacterium-symbiotic fungus Simplicillium lanosoniveum

作者：Dong, Qinglin^*; Dong, Rongzhen; Xing, Xiangying; Li, Yukuan

来源：Natural Product Research, 2018, 32(11): 1348-1352.

DOI：10.1080/14786419.2017.1343320

• Antibiotic
• Simplicillium lanosoniveum
• isolation
• purification
• characterisation

The culture broth of the cyanobacterium-symbiotic fungus Simplicillium lanosoniveum var. Tianjinienss Q. L. Dong exhibited unanticipated antibacterial activities against the Gram-positive bacteria, particularly the pathogenic bacterium Staphylococcus aureus, indicating the secretion of antibiotic-like metabolite, for which the modified Sabouraud medium was the suitable medium. The antibiotic-like metabolite was separated with macroporous resins CT-12 (absorption) and 95% ethanol (desorption), purified by ion-exchange resins D301T and displayed a characteristic absorption peak at 228nm, suggesting the presence of nitrogen. The negative biuret and ninhydrin tests confirmed the absence of -NH2 and -COOH groups. Further, HPLC and mass spectrometry analyses showed that the retention time and molecular weight of the antibiotic-like metabolite were 4.1031min and 163.0182 (+/- 2.3ppm), respectively. Taking together, we speculated that the antibiotic-like metabolite was a new antibiotic structurally similar to alkaloid, which was the first one isolated from the species of Simplicillium genus.
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• 出版日期2018
• 单位河北工业大学