Anti-Pigmentary Effect of (-)-4-Hydroxysattabacin from the Marine-Derived Bacterium Bacillus sp.

作者：Kim Kyuri; Leutou Alain S; Jeong Haein; Kim Dayoung; Seong Chi Nam; Nam Sang Jip^*; Lim Kyung Min^*

来源：Marine Drugs, 2017, 15(5): 138.

DOI：10.3390/md15050138

• Bacillus sp.
• (-)-4-hydroxysattabacin
• melanogenesis
• marine-derived
• anti-pigmentary effect

Bioactivity-guided isolation of a crude extract from a culture broth of Bacillus sp. has led to the isolation of (-)-4-hydroxysattabacin (1). The inhibitory effect of (-)-4-hydroxysattabacin (1) was investigated on melanogenesis in the murine melanoma cell line, B16F10, and human melanoma cell line, MNT-1, as well as a pigmented 3D-human skin model. (-)-4-Hydroxysattabacin treatment decreased melanin contents in a dose-dependent manner in alpha-melanocyte stimulating hormone (alpha-MSH)-stimulated B16F10 cells. Quantitative real time PCR (qRT-PCR) demonstrated that treatment with (-)-4-hydroxysattabacin down-regulated several melanogenic genes, including tyrosinase, tyrosinase-related protein 1 (TRP-1), and tyrosinase-related protein 2 (TRP-2) while their enzymatic activities were unaffected. The anti-melanogenic effects of (-)-4-hydroxysattabacin were further demonstrated in a pigmented 3D human epidermal skin model, Melanoderm (TM), and manifested as whitening and regression of melanocyte activation in the tissue.

• 出版日期2017-5