An analysis of bone regeneration at a segmental bone defect by controlled release of bone morphogenetic protein 2 from a biodegradable sponge composed of gelatin and ss-tricalcium phosphate

作者：Fujita N; Matsushita T; Ishida K; Sasaki K; Kubo S; Matsumoto T; Kurosaka M; Tabata Y; Kuroda R^*

来源：Journal of Tissue Engineering and Regenerative Medicine, 2012, 6(4): 291-298.

DOI：10.1002/term.432

• bone regeneration
• bone morphogenic protein-2
• gelatin sponge
• ss-tricalcium phosphate
• tissue engineering
• scaffold

To treat large bone defects is a clinically challenging problem and utilizing tissue engineering technology is an attractive approach for overcoming such a problem. Previously, a biodegradable sponge incorporating bone morphogenic protein-2 (BMP-2), which can control the release of BMP-2 for a prolonged time in an in vivo environment, was reported. In addition, a biodegradable sponge composed of gelatin and beta-tricalcium phosphate (beta TCP), gelatin-beta TCP sponge to develop a more ideal scaffold for enhancing bone regeneration was also created and previously reported. The purpose of this study was to investigate the effectiveness of the gelatin-beta TCP sponge for the promotion of bone regeneration in a critical-sized bone defect site in vivo. Apparent bone regeneration was induced by the gelatin sponge incorporating BMP-2 and the gelatin-beta TCP sponge with BMP-2 incorporation. In contrast, no apparent bone formation was induced by either the gelatin sponge only or the gelatin-beta TCP sponge without BMP-2. To investigate the quality of the regenerated bone, we conducted a biomechanical evaluation with a three-point bending test. We found no significant difference between the gelatin sponge incorporating BMP-2 and the gelatin-beta TCP sponge incorporating BMP-2 groups. Incorporation of beta TCP into the gelatin sponge was expected to enhance biomechanical strength during the initial bone regeneration. However, our observations showed that the gelatin-beta TCP sponge did not significantly improve the quality of regenerated bone from the viewpoint of biomechanical assessment, even though it did not impair the effectiveness of the promotion of bone regeneration by BMP-2 in the bone defect.

• 出版日期2012-4