Mast Cell-Mediated Antigen Presentation Regulates CD8(+) T Cell Effector Functions

作者：Stelekati Erietta; Bahri Rajia; D'Orlando Orietta; Orinska Zane; Mittruecker Hans Willi; Langenhaun Rabea; Glatzel Markus; Bollinger Annalena; Paus Ralf; Bulfone Paus Silvia^*

来源：Immunity, 2009, 31(4): 665-676.

DOI：10.1016/j.immuni.2009.08.022

The characteristics, importance, and molecular requirements for interactions between mast cells (MCs) and CD8(+) T cells have not been elucidated. Here, we demonstrated that MCs induced antigenspecific CD8(+) T cell activation and proliferation. This process required direct cell contact and MHC class I-dependent antigen cross-presentation by MCs and induced the secretion of interleukin-2, interferon-gamma, and macrophage inflammatory protein-1 alpha by CD8(+) T cells. MCs regulated antigen-specific CD8(+) T cell cytotoxicity by increasing granzyme B expression and by promoting CD8(+) T cell degranulation. Because MCs also upregulated their expression of costimulatory molecules (4-1 BB) and released osteopontin upon direct T cell contact, MC-T cell interactions probably are bidirectional. In vivo, adoptive transfer of antigen-pulsed MCs induced MHC class I-dependent, antigen-specific CD8(+) T cell proliferation, and MCs regulated CD8(+) T cell-specific priming in experimental autoimmune encephalomyelitis. Thus, MCs are important players in antigen-specific regulation of CD8(+) T cells.

• 出版日期2009-10-16