In vitro antimicrobial activity of light-activated phthalocyanines

作者：Ryskova Lenka^*; Buchta Vladimir; Karaskova Marie; Rakusan Jan; Cerny Jiri; Slezak Radovan

来源：Central European Journal of Biology, 2013, 8(2): 168-177.

DOI：10.2478/s11535-013-0118-0

• Phthalocyanines
• Photodynamic antimicrobial therapy
• In vitro photoinactivation
• Staphylococcus aureus
• Escherichia coli
• Candida albicans

Photodynamic antimicrobial therapy (PACT) is proposed as a topical, non-invasive approach suitable for treatment of locally occurring infection. Research of photosensitizers, (PS) as well as their development, is aimed at finding effective antimicrobial substances which would have a broad-spectrum potency. The aim of this paper is to evaluate the antimicrobial effect of phthalocyanine (Pc) derivatives.
Fifteen different Pc compounds were investigated. Their photokilling activity was tested on Staphylococcus aureus, Escherichia coli and Candida albicans. After treating of microbial cells with Pc at the concentrations: 1 mg/l, 2 mg/l, 4 mg/l, 8 mg/l for 30 minutes, the cultures were irradiated with low-power laser light at a wavelength of 670 nm (20 J/cm(2), 40 J/cm(2)). The effectiveness of photoinactivation was evaluated based on the decrease in number (log(10)) of viable bacteria.
Eight Pc compounds tested showed antibacterial effects against S. aureus, but only four were effective against E. coli and two against C. albicans. The most effective photosensitizers were amphiphilic sulphonated zinc Pc compounds [(3-diethylammonium)-propylsulphonamide citrate (Pc3) and cationic tetramethylenepyridinium chloride of hydroxyaluminum Pc (Pc7)].
The most efficient phthalocyanines (Pc3, Pc7) cause a significant decrease in viable counts of all tested microbes.

• 出版日期2013-2