Association of IL28B SNP With Progression of Egyptian HCV Genotype 4 Patients to End Stage Liver Disease

作者：El Awady Mostafa K^*; Mostafa Lotiaf; Tabll Ashraf A; Abdelhafez Tawfeek H; El Din Noha G Bader; Zayed Naglaa; El Shenawy Reem; El Abd Yasmin; Hasan Reham M; Zaghlol Hosam; El Khayat Hesham; Aziz Ashraf O Abdel

来源：Hepatitis Monthly, 2012, 12(4): 271-277.

DOI：10.5812/hepatmon.835

• Hepatitis C
• Interleukin 28B
• Polymorphism, Genetic
• Liver Cirrhosis
• Carcinoma, Hepatocellular

Background: IL28B single nucleotide polymorphisms (SNPs) play important roles in the management of hepatitis C virus (HCV) infections and are strongly associated with spontaneous and treatment-induced HCV clearance. %26lt;br%26gt;Objectives: In the present study, the association between IL28B variants and the progression of HCV infection in Egyptian patients infected with type 4a virus will be examined. %26lt;br%26gt;Patients and Methods: Frequencies of the protective genotype C/C of SNP, rs12979860 were determined in healthy subjects, spontaneous resolvers, and chronic HCV type 4 patients with low F scores and in patients with end stage liver disease (ESLD). This study included a total of 404 subjects. Patients infected with HCV type 4a (n = 304) were divided into; chronic hepatitis C (CHC) with low F scores (CHC, n = 110), end stage liver disease (n = 110), liver cirrhosis (LC) (n = 35) and hepatocellular carcinoma (HCC) patients (n = 75), spontaneous resolvers of HCV infection (n = 84) were also included. A healthy group representing the Egyptian population (n = 100) was also included in the genotyping of IL28B. The later was typed via a polymerase chain reaction based restriction fragment length polymorphism (PCR-RFLP) assay analysis on purified genomic DNA extracted from all individuals. %26lt;br%26gt;Results: A significant increase (P %26lt; 0.0005) was observed in frequencies of IL-28B rs12979860 C/C genotypes in the healthy population, than in the CHC, LC and HCC groups (C/C = 48%, 13%, 0%. and 0% respectively). On the other hand the C/C genotype was significantly higher (P %26lt; 0.0005) in spontaneous resolvers than in healthy subjects. A comparable significant increase in the frequency of C/T allele accompanied by mild elevation of T/T allele frequency, were detected along the progression towards ESLD. %26lt;br%26gt;Conclusions: Genotype C/C is associated with viral clearance during acute infection. The sharp decline in the C/C genotype from healthy to CHC subjects and the total absence of the C/C genotype in ESLD suggests a central role of this genotype against HCV disease progression.

• 出版日期2012-4