Detection of NEO in muskmelon fruits inoculated with Fusarium sulphureum and its control by postharvest ozone treatment

作者：Hua-Li, Xue^*; Yang, Bi^*; Raza, Hussain; Hu-jun, Wang; Lu-Mei, Pu; Mi-Na, Nan; Xiao-Yan, Cheng; Yi, Wang; Yong-Cai, Li

来源：Food Chemistry, 2018, 254: 193-200.

DOI：10.1016/j.foodchem.2018.01.149

• Muskmelon
• Fusarium sulphureum
• Trichothecenes
• Ozone
• Control

Fusarium rot of muskmelon, caused by Fusarium spp., is one of the most important postharvest decays, that not only causes economic losses but leads to trichothecenes contamination. A rapid and sensitive method was developed for neosolaniol (NEO) analysis in muskmelon inoculated with F. sulphureum, utilizing acetonitrile/water (84:16, v/v) extraction and PriboFast M270 columns purification and UPLC-MS/MS detection. Method validation was evaluated by linearity (R >= 0.9990), recovery (88.1-136.9%), precision (RSD <= 3.97%) and sensitivity (LOD, 0.5 mu g/kg; LOQ, 1.5 mu g/kg). The effect of ozone treatment on Fusarium rot development and NEO accumulation in inoculated muskmelon was also evaluated. The results showed that UPLC-MS/MS method was suitable for analyzing NEO in inoculated muskmelon, and 1.10 mg/l ozone treatment for 120 min significantly controlled Fusarium rot development and NEO accumulation in fruits after 5, 8 and 11 days. In vivo tests showed that ozone at 1.10 mg/l effectively degraded NEO in acetonitrile.

• 出版日期2018-7-15
• 单位甘肃农业大学