Detection of chitinase ChiA produced by Serratia marcescens PRC-5, using anti-PrGV-chitinase

作者：Song Yong Su; Oh Seunghan; Han Yeon Soo; Seo Dong Jun; Park Ro Dong; Jung Woo Jin^*

来源：Carbohydrate Polymers, 2013, 92(2): 2276-2281.

DOI：10.1016/j.carbpol.2012.12.018

• Chitinolytic activity
• Chitinase isozyme
• Anti-PrGV-chitinase
• Rhizoctonia solani

In this study, a bacterium Serratia marcescens PRC-5 that displayed strong chitinolytic activity on 0.5% colloidal chitin-containing agar medium was isolated from soil. The chitinase activity increased rapidly with a maximum level (6.14 U/mL) on 4 days of incubation with swollen chitin (pH 5.0). Three active bands of chitinase isozymes were observed (53, 44, and 34 kDa) on SDS-PAGE gel and there pI values ranged from pI 5.4 to 5.8 on 2D gels. The chitinase from the PRC-5 strain was also able to produce GlcNAc monomers on TLC plates. The chitinase of PRC-5 inhibited the mycelial growth of Rhizoctonia solani KACC40111, which indicates that it could be used as a biocontrol agent for phytophathogens. The chitinase isozyme N1, which had a molecular weight of 62 kDa, was transferred from a native and SDS-PAGE gel onto an immunoblot and was probed using an anti-PrGV-chitinase.

• 出版日期2013-2-15