Application of Gold Nanoparticles and L-Cysteine Double Layer on Commercial Thin-Layer Chromatography Plates as a New Substrate for Direct Resolution of Propranolol Enantiomers

作者：Absalan Ghodratollah^*; Akhond Morteza; Rafatmah Elmira; Alipour Yousef

来源：Journal of Planar Chromatography - Modern TLC, 2014, 27(6): 409-415.

DOI：10.1556/JPC.27.2014.6.1

• Gold nanoparticles
• L-Cysteine
• Propranolol
• Enantioseparation
• Thin-layer chromatography

Gold nanoparticles (AuNPs) and L-cysteine (L-cys), in order, as first and second layer were coated on the surface of a commercial thin-layer chromatography (TLC) plate. This assemble has been used as a new substrate for direct resolution of propranolol enantiomers based on the ordinary TLC technique. The effect of concentrations of the involved chemicals, time periods of the required processes, pH of the sample solutions, as well as the effects of different coating protocols on the resolution of the enantiomers, were investigated in order to find the optimized separation conditions. The results showed that 10.0 mM copper(II) acetate, in 70% ethanol-water adjusted at pH = 6.3, was suitable for being used as the mobile phase. The AuNPs with the average size of 15 nm and L-cys solution with a concentration of 10.0 mM and pH = 8.1 had been chosen for impregnating TLC plates.

• 出版日期2014-12