科研之友
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摘要
Bursicon is a cystine knot glycoprotein hormone that is composed of two subunits; alpha and beta. Bursicon plays several physiological roles in insects and crustaceans such as cuticle tanning, wing expansion and molting process. Because of its structural similarity with vertebrate gonadotropins, this study therefore aimed to investigate a novel function of bursicon in vitellogenin (Vg) stimulation in reproductive female shrimp, Penaeus monodon. Full-length cDNAs encoding the alpha and beta subunits of bursicon in P. monodon (Pmburs alpha and Pmburs beta) were obtained by rapid-amplification of cDNA ends. Both Pmbursa and Pmburs beta mRNA levels were dominantly expressed in subesophageal ganglia and thoracic ganglia. The expression of Pmburs alpha and Pmburs beta in subesophageal ganglia was significantly increased in early-vitellogenic stage of ovarian development suggesting a possible role of bursicon in vitellogenesis. To study the function of bursicon in shrimp reproduction, recombinant Pmburs alpha (rburs alpha), Pmburs beta (rburs beta) and Pmburs alpha beta (rburs alpha beta) were successfully expressed in the methylotrophic yeast, Pichia pastoris. A treatment of shrimp ovarian cells with recombinant Pmburs showed that only rburs alpha beta could induce the expression of Vg mRNA. In addition, injection of rburs alpha beta into female P. monodon broodstock caused an increase of Vg mRNA expression in the ovary as well as stimulated ovarian development. This study provides the first evidence for a reproductive role, most likely as an invertebrate neuropeptide gonad-stimulating hormone, of the heterodimeric bursicon in P. monodon. Statement of relevance Useful information for improvement of shrimp reproduction.
- 出版日期2015-9-1
