科研之友
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摘要
A new type of rapid, sensitive, and selective fluorescence turn-on assay was developed for detection of histidine using a DNA-scaffolded silver nanocluster/Cu2 ensemble (DNA-AgNC/Cu2 ). Cu2 was first bound to nucleic acids of the DNA-AgNCs forming a DNA-AgNC/Cu2 ensemble and then liberated into solution via the highly specific interaction between histidine and Cu2 in the presence of other amino acids. The fluorescence of DNA-AgNCs was greatly quenched with the addition of Cu2 , and then the DNA-AgNC/Cu2 ensemble exhibited marvelous fluorescent enhancement in the presence of histidine, which showed the possibility for constructing a turn-on chemosensor of histidine. Compared to other methods, this approach promises high sensitivity, simplicity in design, convenient operation, and minimized use of organic solvents. The ultra-high selectivity demonstrated the feasibility of the assay for detecting histidine in sophisticated physical environments. The fitting range of the proposed assay is from 0 to 100 mu M, with a detection limit of 1.4 mu M (S/N=3) in artificial urine samples. The protocol was evaluated by analysis of artificial urine samples with good recoveries and showed great potential for practical applications.
- 出版日期2014
- 单位华东师范大学
