Molecular Characterization and Expression of As-nurp1 Gene From Artemia sinica During Development and in Response to Salinity and Temperature Stress

作者：Li, Qiuying; Zhang, Qiaozhi; Han, Lulu; Yuan, Zhe; Tan, Jian; Du, Bin; Zou, Xiangyang^*; Hou, Lin

来源：The Biological Bulletin, 2012, 222(3): 182-191.

DOI：10.1086/BBLv222n3p182

• BREAST-CANCER CELLS
• P8 MESSENGER-RNA
• PROTEIN P8
• NUCLEAR-PROTEIN
• BINDING-PROTEIN
• CLONING
• TRANSCRIPTION
• EMBRYOS
• TOLERANCE
• APOPTOSIS

Nuclear protein 1 (NURP1) is a stress-related protein and closely related to diapause in the development of Artemia. In the present paper, the full-length 568-bp cDNA sequence of the nurp1 homolog of Artemia sinica (As-nurp1) was isolated by RACE technology for the first time. The putative As-nurp1 protein consists of 66 amino acids with a basic helix-loop-helix (bHLH) motif and a bipartite nuclear localization signal (NLS). Multiple sequence alignments revealed that the putative As-nurp1 protein sequence was relatively conserved across species, especially in the bHLH domain. The expression of As-nurp1 is widely distributed during A. sinica development. This is followed by a dramatic downregulation after diapause and is newly upregulated from the larval nauplius stage. Furthermore, As-nurp1 transcripts are highly upregulated under conditions of high salinity and low temperature. These findings suggest that As-nurp1 is stress-related and may act as a protective factor in embryonic development.

• 出版日期2012-6
• 单位辽宁师范大学; 大连医科大学