Development of microwave assisted spectrophotometric method for the determination of glucose

作者：Ali Asif; Hussain Zahid; Arain Muhammad Balal^*; Shah Nasrullah; Khan Khalid Mohammad; Gulab Hussain; Zada Amir

来源：Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy , 2016, 153: 374-378.

DOI：10.1016/j.saa.2015.07.104

• Microwave assisted analysis
• Maillard product analysis
• Glucose
• Glycine coupling

A spectrophotometric method was developed based on the microwave assisted synthesis of Maillard product. Various conditions of the reaction were optimized by varying the relative concentration of the reagents, operating temperature and volume of solutions used in the reaction in the microwave synthesizer. The absorbance of the microwave synthesized Maillard product was measured in the range of 360-740 nm using UV-Visible spectrophotometer. Based on the maximum absorbance, 370 nm was selected as the optimum wave length for further studies. The LOD and LOQ of glucose was found 3.08 pg mL(-1) and 9.33 mu g mL(-1) with standard deviation of +/- 0.05. The developed method was also applicable to urine sample.

• 出版日期2016-1-15