Adaptive laboratory evolution resolves energy depletion to maintain high aromatic metabolite phenotypes in Escherichia coli strains lacking the Phosphotransferase System

作者：McCloskey Douglas; Xu Sibei; Sandberg Troy E; Brunk Elizabeth; Hefner Ying; Szubin Richard; Feist Adam M; Palsson Bernhard O^*

来源：Metabolic Engineering, 2018, 48: 233-242.

DOI：10.1016/j.ymben.2018.06.005

• Adaptive laboratory evolution
• Mutation analysis
• Multi-omics analysis
• Systems biology
• E. coli
• ptsH
• ptsl
• crr gene knockouts

Aromatic metabolites provide the backbone for numerous industrial and pharmaceutical compounds of high value. The Phosphotransferase System (PTS) is common to many bacteria, and is the primary mechanism for glucose uptake by Escherichia coll. The PTS was removed to conserve phosphoenolpyruvate (pep), which is a precursor for aromatic metabolites and consumed by the PTS, for aromatic metabolite production. Replicate adaptive laboratory evolution (ALE) of PTS and detailed omics data sets collected revealed that the PTS bridged the gap between respiration and fermentation, leading to distinct high fermentative and high respiratory rate phenotypes. It was also found that while all strains retained high levels of aromatic amino acid (AAA) biosynthetic precursors, only one replicate from the high glycolytic Glade retained high levels of intracellular AAAs. The fast growth and high AAA precursor phenotypes could provide a starting host for cell factories targeting the overproduction aromatic metabolites.

• 出版日期2018-7