Assisted reproductive technologies in the common marmoset: an integral species for developing nonhuman primate models of human diseases

作者：Kropp Jenna; Di Marzo Andrea; Golos Thaddeus^*

来源：Biology of Reproduction, 2017, 96(2): 277-287.

DOI：10.1095/biolreprod.116.146514

• in vitro fertilization
• marmoset
• transgenesis
• genomic editing

Generation of nonhuman primate models of human disease conditions will foster the development of novel therapeutic strategies. Callithrix jacchus, or the common marmoset, is a NewWorld, nonhuman primate species that exhibits great reproductive fitness in captivity with an ovarian cycle that can be easily managed with pharmacological agents. This characteristic, among others, provides an opportunity to employ assisted reproductive technologies to generate embryos that can be genetically manipulated to create a variety of nonhuman primate models for human disease. Here, we review methods to synchronize the marmoset ovarian cycle and stimulate oocyte donors, and compare various protocols for in vitro production of embryos. In light of advances in genomic editing, recent approaches used to generate transgenic or genetically edited embryos in the marmoset and also future perspective are reviewed.

• 出版日期2017-2