Application of phage display to high throughput antibody generation and characterization

作者：Schofield Darren J; Pope Anthony R; Clementel Veronica; Buckell Jenny; Chapple Susan D J; Clarke Kay F; Conquer Jennie S; Crofts Anna M; Crowther Sandra R E; Dyson Michael R; Flack Gillian; Griffin Gareth J; Hooks Yvette; Howat William J; Kolb Kokocinski Anja; Kunze Susan; Martin Cecile D; Maslen Gareth L; Mitchell Joanne N; O'Sullivan Maureen; Perera Rajika L; Roake Wendy; Shadbolt S Paul; Vincent Karen J; Warford Anthony; Wilson Wendy E; Xie Jane

来源：Genome Biology, 2007, 8(11): R254.

DOI：10.1186/gb-2007-8-11-r254

We have created a high quality phage display library containing over 1010 human antibodies and describe its use in the generation of antibodies on an unprecedented scale. We have selected, screened and sequenced over 38,000 recombinant antibodies to 292 antigens, yielding over 7,200 unique clones. 4,400 antibodies were characterized by specificity testing and detailed sequence analysis and the data/clones are available online. Sensitive detection was demonstrated in a bead based flow cytometry assay. Furthermore, positive staining by immunohistochemistry on tissue microarrays was found for 37% (143/381) of antibodies. Thus, we have demonstrated the potential of and illuminated the issues associated with genome-wide monoclonal antibody generation.

• 出版日期2007