A comparative analysis of genetic diversity in medicinal Chrysanthemum morifolium based on morphology, ISSR and SRAP markers

作者：Shao, Qing-Song; Guo, Qiao-Sheng^*; Deng, Yan-Ming; Guo, Hai-Peng

来源：Biochemical Systematics and Ecology, 2010, 38(6): 1160-1169.

DOI：10.1016/j.bse.2010.11.002

• Chrysanthemum morifolium
• Genetic diversity
• Morphological traits
• ISSR
• SRAP

The diversity and genetic relationship among 29 populations of Chrysanthemum morifolium, one of Chrysanthemum indicum and one of Chrysanthemum nankingense from China were analyzed using morphological traits and molecular markers. Twenty morphological traits were scored as well as 182 ISSR marker-fragments, as amplified by 22 primers (the percentage of polymorphic bands (PPB): 81.87%], and 243 SRAP marker-fragments as generated by 26 primer pairs (PPB: 75.72%). Mantel's test indicated significant correlation (r = 0.624) of morphological trait and SRAP. By contrast, the morphological trait showed low correlation with ISSR (r = 0.246). Cluster analysis showed groupings of the accessions according to all four methods correlated well with their geographic region of origin, and most populations from the south of China were classified into one cluster and most populations from the north of China were classified into another cluster. Finally, an appropriate strategy for conserving the C. morifolium germplasm was proposed.

• 出版日期2010-12
• 单位浙江农林大学; 江苏省农业科学院; 南京农业大学