Antioxidant and Cytoprotective Effects of Kukoamines A and B: Comparison and Positional Isomeric Effect

作者：Li, Xican^*; Lin, Jian; Chen, Ban; Xie, Hong; Chen, Dongfeng^*

来源：Molecules, 2018, 23(4): 973.

DOI：10.3390/molecules23040973

• positional isomeric effect
• antioxidant mechanisms
• cytoprotective effect
• kukoamine A
• kukoamine B
• phenolic polyamine

In this study, two natural phenolic polyamines, kukoamine A and B, were comparatively investigated for their antioxidant and cytoprotective effects in Fenton-damaged bone marrow-derived mesenchymal stem cells (bmMSCs). When compared with kukoamine B, kukoamine A consistently demonstrated higher IC50 values in PTIO center dot-scavenging (pH 7.4), Cu2+-reducing, DPPH center dot-scavenging, center dot O-2 -scavenging, and center dot OH-scavenging assays. However, in the PTIO center dot-scavenging assay, the IC50 values of each kukoamine varied with pH value. In the Fe2+-chelating assay, kukoamine B presented greater UV-Vis absorption and darker color than kukoamine A. In the HPLC-ESI-MS/MS analysis, kukoamine A with DPPH center dot produced radical-adduct-formation (RAF) peaks (m/z 922 and 713). The 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl (MTT) assay suggested that both kukoamines concentration-dependently increased the viabilities of Fenton-damaged bmMSCs at 56.5-188.4 mu M. However, kukoamine A showed lower viability percentages than kukoamine B. In conclusion, the two isomers kukoamine A and B can protect bmMSCs from Fenton-induced damage, possibly through direct or indirect antioxidant pathways, including electron-transfer, proton-transfer, hydrogen atom transfer, RAF, and Fe2+-chelating. Since kukoamine B possesses higher potentials than kukoamine A in these pathways, kukoamine B is thus superior to kukoamine A in terms of cytoprotection. These differences can ultimately be attributed to positional isomeric effects.

• 出版日期2018-4
• 单位广州中医药大学