Nepeta japonica Maximowicz extract from natural products inhibits lipid accumulation

作者：Roh Changhyun^*; Jung Uhee

来源：Journal of the Science of Food and Agriculture, 2012, 92(10): 2195-2199.

DOI：10.1002/jsfa.5608

• anti-obesity
• lipid inhibition
• natural products
• Nepeta japonica Maximowicz

BACKGROUND: To develop novel and crude anti-obesity drugs from natural products is a promising field to approach the solution to a global health problem such as obesity. The aim of this study was to screen crude anti-obesity drugs from 400 natural products on lipase inhibition activity in vitro. RESULTS: Among the natural products examined, 31 extracts showed significantly inhibition activity against porcine pancreatic lipase (triacylglycerol lipase, EC 3.1.1.3) by using spectrophotometry with 2,4-dinitrophenylbutyrate as a substrate. Furthermore, 31 natural products were investigated with regard to their lipid inhibition in 3T3-L1 cells. Among these, one of most promising was Nepeta japonica Maximowicz extract, which showed inhibition of triglyceride accumulation in 3T3-L1 adipocytes, suggesting anti-obesity activity. Also, the amount of glycerol released from cells into the medium was increased by treatment of Nepeta japonica Maximowicz extract at a concentration of 100 mu g mL-1. CONCLUSION: The present study suggests that a promising crude anti-obesity drug screened from 400 natural products might be of therapeutic interest with respect to the treatment of obesity.

• 出版日期2012-8