Acinetobacter halotolerans sp nov., a novel halotolerant, alkalitolerant, and hydrocarbon degrading bacterium, isolated from soil

作者：Dahal Ram Hari; Chaudhary Dhiraj Kumar; Kim Jaisoo^*

来源：Archives of Microbiology, 2017, 199(5): 701-710.

DOI：10.1007/s00203-017-1349-2

• Acinetobacter halotolerans sp nov.
• Halotolerant
• Alkalitolerant
• Hydrocarbon degrading bacteria

A novel aerobic, non-motile, halotolerant, alkalitolerant, hydrocarbon degrading, and rod shaped bacterium, designated strain R160(T), was isolated from soil in South Korea. Cells were Gram-staining-negative, catalase-positive, and oxidase-negative. This strain grew up to 7% of NaCl and in the pH range of 6-11 (optimum 7.0-10.0). The isolate degraded 51.7 +/- 1.3% of hydrocarbon components (C-18, C-20, and C-22) and 45.8 +/- 1.4% oil components (kerosene, diesel, and gasoline). Phylogenetic analysis based on 16 S rRNA gene sequences revealed that strain R160(T) formed a lineage within the genus Acinetobacter, and was closely related to 'Acinetobacter oleivorans' DR1(T) (97.47%, sequence similarity). Other closely related members have sequence similarity between 97.47 to 96.52%. The predominant respiratory lipoquinones of strain R160(T) were ubiquinone 9 (Q-9) and ubiquinone 8 (Q-8). The major polar lipids were phosphatidylethanolamine (PE), diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), and phosphatidylcholine (PC). The major cellular fatty acids were 9-octadecenoic acid (C-18:1 omega 9c), hexadecanoic acid (C-16:0), and summed feature (comprising C-16:1 omega 7c and/or C-16:1 omega 6c). The DNA G + C content of strain R160(T) was 44.9 mol%. On the basis of phenotypic, genotypic, chemotaxonomic, and phylogenetic characteristics, strain R160(T) represents a novel species of the genus Acinetobacter, for which the name Acinetobacter halotolerans sp. nov. is proposed. The type strain is R160(T) (= KEMB 9005-333(T) = KACC 18453(T) = JCM 31009(T)).

• 出版日期2017-7