Development of a Competitive Indirect Enzyme-Linked Immunosorbent Assay for Screening Phenylethanolamine A Residues in Pork Samples

作者：Wang, Xiaomei; Liufu, Tongling; Beloglazova, Natalia V.; Luo, Pengjie; Qu, Jianwen; Jiang, Wenxiao^*

来源：Food Analytical Methods, 2016, 9(11): 3099-3106.

DOI：10.1007/s12161-016-0500-z

• Phenylethanolamine A
• Antibody
• Pork
• Residue
• Food safety
• ciELISA

Phenylethanolamine A (PEA), a new alternative beta-agonist, has been illegally used in farming to promote the muscle growth in food-producing animals. In this study, a sensitive and convenient competitive indirect enzyme-linked immunosorbent assay (ciELISA) was developed for determination of PEA residues in pork samples. The produced antibody was highly specific to PEA and exhibited a negligible cross-reactivity toward some other beta-agonists. The developed technique was characterized by the limit of detection below 0.08 mu g kg(-1) and the IC50 value of 0.93 pmol mL(-1) (0.32 ng mL(-1)). Validation of the technique was done using artificially spiked and naturally contaminated pork samples. The recoveries ranged from 79.6 to 112.6 % for the samples spiked at levels of 0.1-5 mu g kg(-1) with the variation coefficients below 15 %. The analysis of naturally contaminated samples showed that the obtained data corresponded with the data obtained by the LC-MS/MS. The developed ciELISA was shown to be a feasible highly sensitive and specific screening tool for PEA residue analysis.

• 出版日期2016-11
• 单位国家食品安全风险评估中心; 深圳大学