Annexin V Release and Transmembrane Mitochondrial Potential during Storage of Apheresis-Derived Platelets Treated for Pathogen Reduction

作者：Picker Susanne M^*; Oustianskaia Larissa; Schneider Volker; Gathof Birgit S

来源：Transfusion Medicine and Hemotherapy, 2010, 37(1): 7-12.

DOI：10.1159/000264666

• Pathogen reduction technology
• Platelet in vitro function
• Endogenous annexin V
• Transmembrane mitochondrial potential
• INTERCEPT BLOOD SYSTEM
• MIRASOL-PRT

Background: In vitro function of stored platelet (PLT) concentrates was analyzed after applying two different techniques of pathogen reduction technology (PRT) treatment, which could increase cellular injury during processing and storage. Methods: Nine triple-dose PLT apheresis donations were split into 27 single units designated to riboflavin-UVB (M) or psoralen-UVA (I) treatment or remained untreated (C). Throughout 8 days of storage, samples were analyzed for annexin V release, the mitochondrial transmembrane potential (Delta psi) and some classical markers of PLT quality (pH, LDH release, hypotonic shock response (HSR)). Results: PLT count and LDH release of all units maintained initial ranges. All units exhibited a decrease in pH and HSR and an increase in annexin V release and Delta psi disruption. Notably, throughout the entire storage period, annexin V release remained lowest in M units. Throughout 7 days of storage, M units remained comparable to C units (p > 0.05), whereas inferior values were observed with I units. Here, differences to C units reached significance by day 1 (pH: p < 0.0001), day 5 (annexin V release: p < 0.014), and day 7 (HSR, Delta psi: p <= 0.003). After PRT treatment, annexin V release and Delta psi disruption were significantly (p < 0.001) correlated with pH and HSR. Conclusion: During storage, all units showed a decrease in HSR and an increase in acidity, annexin V release and Delta psi disruption. While M units remained comparable to C units, I units demonstrated significantly inferior values during terminal storage. This could have resulted from differences in PRT treatment or simply be due to differences in storage media and should be analyzed for clinical relevance in future investigations.

• 出版日期2010