Stability study for magnetic reagent assaying Hb and HbA1c

作者：Hsieh Wen Pin; Chieh J J; Yang C C; Yang S Y; Chen Po Yu; Huang Yu Hao; Hong Y W; Horng H E^*

来源：Journal of Magnetism and Magnetic Materials, 2013, 326: 147-150.

DOI：10.1016/j.jmmm.2012.08.029

• Magnetic particle
• Immunoassay
• Hb
• HbA1c
• Stability

Reagents for magnetically labeled immunoassay on human Hb and human HbA1c have been synthesized. The reagents consist of Fe3O4 magnetic particles biofunctionalized with antibodies against Hb and HbA1c. It has been demonstrated that the reagents can be applied to quantitatively detect Hb and HbA1c by using immunomagnetic reduction assay. In addition to characterizing the assay properties, such as the standard curve and the low-detection limit, the stability of reagents is investigated. To do this, the temporal dependence of particle sizes and the bio-activity of reagents are monitored. The results show that the reagents are highly stable when stored at 2-8 degrees C. This means that the reagents synthesized in this work are promising for practical applications.

• 出版日期2013-1