Acute Respiratory Distress Syndrome Induced by White Smoke Inhalation: a Potential Animal Model For Evaluating Pathological Changes and Underlying Mechanisms

作者：Cui, Pei; Feng, Xiaoyan; Zhu, Feng; Yao, Yongming; Xiao, Shichu; Gong, Zhenyu; Xin, Haiming; Zhan, Qiu; Yang, Fuwang; Deng, Chunjiang; Tang, Zhiping; Tong, Yalin^*; Xia, Zhaofan^*

来源：Cellular Physiology and Biochemistry, 2018, 47(6): 2396-2406.

DOI：10.1159/000491614

• White smoke inhalation
• Acute respiratory distress syndrome
• NF-kappa B
• Therapeutic target
• Animal model

Background/Aims: White smoke inhalation (WSI) is an uncommon but potentially deadly cause of acute respiratory distress syndrome (ARDS). However, no clinical treatment protocol has been established for the treatment of WSI-induced ARDS. Therefore, it is necessary to investigate the effects of WSI in ARDS and the mechanisms underlying the effects of WSI to determine a novel therapeutic target. Methods: On the basis of the duration of continued inhalation of white smoke (3 min, 5 min, and 7 min), rats were divided into three groups (WSI3 min, WSI-5 min, and WSI-7 min). The survival rate, pathological change, and computed tomography (CT) score were evaluated to determine the modeling conditions. In the established WSI-5 min models, evaluations were performed to evaluate the following: arterial blood gas levels, lung wet/dry weight ratio, the expression of inflammatory cytokines, and the effect of NF-kappa B signaling pathway. Results: The survival rate of rats at 72 h post-WSI in the WSI-3 min, WSI-5 min, and WSI-7 min groups was 83.33%, 75%, and 25%, respectively. Results from evaluation of H&E staining, CT scan, arterial blood gas levels, and lung wet/dry weight ratio suggest that the pathological changes in the rat in the WSI-5 min and WSI-7 min groups are very similar to those in patients with ARDS induced by WSI. Additionally, the expression of INF-gamma, TGF-beta 1, TNF-alpha, and IL-1 beta were increased, and the NF-kappa B signaling pathway was activated in the WSI-5 min group. Conclusion: The rat model of WSI-5 min can be used as a WSI-induced ALI model for further experiments. The NF-kappa B signaling pathway may be a potential therapeutic target for the treatment of WSI-induced ARDS.

• 出版日期2018
• 单位中国人民解放军第一八一医院