A particular expression pattern of CD13 epitope 7H5 in chronic lymphocytic leukaemia - a possible new therapeutic target

作者：Guenova Margarita L^*; Michova Antoaneta; Balatzenko Gueorgui N; Yosifov Deyan Y; Stoyanov Nikolay; Taskov Hristo; Berger Martin R; Konstantinov Spiro M

来源：Hematology, 2012, 17(3): 132-139.

DOI：10.1179/102453312X13376952196296

• Aminopeptidase N
• CD13
• Chronic lymphocytic leukaemia
• Curcumin
• Apoptosis
• Cytoreductive activity

A total of 50 patients with chronic lymphocytic leukaemia (CLL), as well as the B-cell leukaemia cell lines MEC-1, JVM-3, and BV-173 were studied in order to assess the incidence of CD13/aminopeptidase N (APN) immunolabelling with a monoclonal antibody 7H5 compared to LeuM7 and to CD13 mRNA levels, and to correlate these data with the cytotoxic and apoptosis-induction activity of the natural phenolic APN inhibitor curcumin. CD13/APN was detected in a significant proportion of B-CLL patients (42/50, 84%), immunolabelled by 7H5 (42/50) +/- LeuM7 (10/50). Molecular analysis for CD13 transcripts confirmed these data, resulting in a specific RT-PCR product in CD13 positive cases. Curcumin showed concentration-dependent cytoreductive efficacy and apoptosis-induction activity in all tested cell lines and primary cultures from CLL mononuclear cells. There was a clear tendency for a better response in CD13 positive cases. The incidence of CD13/APN in CLL suggests that the inhibition of APN/CD13 by curcumin may be an effective new molecular target for a more efficient therapy for these patients and warrants further investigations.

• 出版日期2012-5