Measurement of cytoplasmic Ca2+concentration in Saccharomyces cerevisiae induced by air cold plasma

作者：Dong, Xiaoyu^*

来源：Plasma Science and Technology, 2018, 20(4): 044001.

DOI：10.1088/2058-6272/aa9479

• cytoplasmic Ca2+ concentration
• Saccharomyces cerevisiae
• air cold plasma
• parameter optimization

In this study, a novel approach to measure the absolute cytoplasmic Ca2+ concentration ([Ca2+] cyt) using the Ca2+ indicator fluo-3 AM was established. The parameters associated with the probe fluo-3 AM were optimized to accurately determine fluorescence intensity from the Ca2+-bound probe. Using three optimized parameters (final concentration of 6 mM probe, incubation time of 135 min, loading probe before plasma treatment), the maximum fluorescence intensity (F-max = 527.8 a.u.) and the minimum fluorescence intensity (F-min = 63.8 a.u.) were obtained in a saturated Ca2+ solution or a solution of lacking Ca2+ Correspondingly, the maximum [Ca2+] cyt induced by cold plasma was 1232.5 nM. Therefore, the Ca2+ indicator fluo-3 AM was successfully applied to measure the absolute [Ca2+] cyt in Saccharomyces cerevisiae stimulated by cold plasma at atmospheric air pressure.

• 出版日期2018-4
• 单位大连大学