Age-Dependent Association between Pulmonary Tuberculosis and Common TOX Variants in the 8q12-13 Linkage Region

作者：Grant Audrey V; El Baghdadi Jamila^*; Sabri Ayoub; El Azbaoui Safa; Alaoui Tahiri Kebir; Rhorfi Ismail Abderrahmani; Gharbaoui Yasser; Abid Ahmed; Benkirane Majid; Raharimanga Vaomalala; Richard Vincent; Orlova Marianna; Boland Anne; Migaud Melanie; Okada Satoshi; Nolan Daniel K; Bustamante Jacinta; Barreiro Luis B; Schurr Erwin; Boisson Dupuis Stephanie; Rasolofo Voahangy; Casanova Jean Laurent; Abel Laurent

来源：American Journal of Human Genetics, 2013, 92(3): 407-414.

DOI：10.1016/j.ajhg.2013.01.013

Only a small fraction of individuals infected with Mycobacterium tuberculosis develop clinical tuberculosis (TB) in their lifetime. Genetic epidemiological evidence suggests a genetic determinism of pulmonary TB (PTB), but the molecular basis of genetic predisposition to PTB remains largely unknown. We used a positional-cloning approach to carry out ultrafine linkage-disequilibrium mapping of a previously identified susceptibility locus in chromosomal region 8q12-13 by genotyping 3,216 SNPs in a family-based Moroccan sample including 286 offspring with PTB. We observed 44 PTB-associated SNPs (p %26lt; 0.01), which were genotyped in an independent set of 317 cases and 650 controls from Morocco. A single signal, consisting of two correlated SNPs close to TOX, rs1568952 and rs2726600 (combined p = 1.1 x 10(-5) and 9.2 x 10(-5), respectively), was replicated. Stronger evidence of association was found in individuals who developed PTB before the age of 25 years (combined p for rs1568952 = 4.4 x 10(-8); odds ratio of PTB for AA versus AG/GG = 3.09 [1.99-4.78]). The association with rs2726600 (p = 0.04) was subsequently replicated in PTB-affected subjects under 25 years in a study of 243 nuclear families from Madagascar. Stronger evidence of replication in Madagascar was obtained for additional SNPs in strong linkage disequilibrium with the two initial SNPs (p = 0.003 for rs2726597), further confirming the signal. We thus identified around rs1568952 and rs2726600 a cluster of SNPs strongly associated with early-onset PTB in Morocco and Madagascar. SNP rs2726600 is located in a transcription-factor binding site in the 3%26apos; region of TOX, and further functional explorations will focus on CD4 T lymphocytes.

• 出版日期2013-3-7
• 单位中国地震局