Xia-Bai-San inhibits lipopolysaccharide-induced activation of intercellular adhesion molecule-1 and nuclear factor-kappa B in human lung cells

作者：Lee Kuo Hua; Yeh Ming Hsien; Kao Shung Te; Hung Che Ming; Chen Bor Chyuan; Liu Ching Ju; Yeh Chia Chou^*

来源：Journal of Ethnopharmacology, 2009, 124(3): 530-538.

DOI：10.1016/j.jep.2009.05.007

• Xia-Bai-San
• Lipopolysaccharide
• Inflammatory cytokine
• ICAM-1
• NF-kappa B
• Lung cell

Xia-Bai-San (XBS) is a traditional Chinese medicine that has been used clinically for centuries in Asian countries to treat some types of common cold and asthma-like diseases similar to infantile pneumonia and childhood bronchitis. In previous studies, XBS was found to suppress the inflammatory process induced in lungs of mice treated with lipopolysaccharide (LPS).
Purpose: The present study was undertaken to examine the effects of XBS on LPS-inducible production of inflammatory cytokines, up-regulation of intercellular cell adhesion molecule-1 (ICAM-1), and activation of nuclear factor NF-kappa B in cultured human lung cells.
Principal results: Extracts of four raw herbs (Cortex mori, Cortex lycii, Radix glycyrrhizae, and Fructus oryzae) were used to prepare the decoction. XBS decreased the histological damage and up-regulation of ICAM-1 observed in lungs of mice treated with lipopolysaccharide (LPS). In cultured human pulmonary epithelial A549 cells, XBS and its components Mortis alba and Glycyrrhiza uralensis suppressed the up-regulation of IL-8 and ICAM-1 in response to LPS. Production of TNF-alpha, IL-1 beta, IL-6 and IL-8 by LPS-treated human THP-1 monomyelocytes was also suppressed by XBS. A549 cells expressed ICAM-1 in response to medium from LPS-treated THP-1 cells: expression was decreased by XBS. The adhesion of THP-1 cells to LPS-treated A549 cells were inhibited in the presence of XBS. Activation of NF-kappa B by LPS in A549 cells was suppressed by XBS, Morus alba, and Glycyrrhiza uralensis through inhibition Of I kappa B phosphorylation; the concentrations at which suppression occurred were identical to those at which production of inflammatory cytokines and up-regulation of ICAM-1 were inhibited.
Major conclusions: These findings support the hypothesis that XBS, Morus alba, and Glycyrrhiza uralensis inhibit the inflammatory process in lung tissue through suppression of the I kappa B signaling pathway. XBS may prove helpful in the management of asthma. various allergic disorders, sepsis, or any other condition associated with pulmonary inflammation.

• 出版日期2009-7-30
• 单位中国医科大学