摘要
Growth of animal is largely regulated by Growth Hormone (GH). In this study the objective was to investigate variations in the fifth exon of GH gene and their polymorphism in 5 Chinese indigenous donkeys by PCR-SSCP and DNA Sequencing Methods. The results showed that there was a transversion at nucleotide position 1802 of GH gene in the 5th exon named as GH-exon5-G1802C which led to a conservative Lysine to Asparagine substitution at amino acid position 205. In detail, two different alleles, A and B were identified and three genotypes were observed, AA, AB and BB with the frequency distribution of allele B from 0.5962-0.7344 in analyzed populations. The genetic diversity analysis revealed that all PIC values were between 0.3140 and 0.3656, implying that this locus within GH gene possessed moderate genetic diversity in 5 Chinese indigenous donkeys. The chi(2)-test showed that GZ donkey was significant deviation, DZ donkey was no significant deviation from Hardy-Weinberg equilibrium, GL, TN and XJ donkey was not in agreement Hardy-Weinberg equilibrium. The results confirmed that there were polymorphisms in the exon 5 of GH gene.
- 出版日期2012
- 单位河北科技师范学院