Augmented humoral and cellular immune responses induced by canine adenovirus type 1 DNA vaccine in BALB/c mice

作者：Wang Jun Xia; Zheng Long; Song Shu Xia; Zhang Xia; Li Li Min; Wang Fang; Liu Ying Fu; Sun Shu Han^*

来源：Viral Immunology, 2007, 20(3): 461-467.

DOI：10.1089/vim.2007.0018

Infectious canine hepatitis (ICH) is caused by canine adenovirus type 1 (CAV-1), which severely harms infected animals. Vaccination provides an effective approach to preventing canine infectious diseases. With the objective of exploring a new vaccination strategy that may prevent or cure ICH, we constructed a DNA vaccine, pVAX1-CpG-Loop, and evaluated its immune efficacy. We found that vaccination of BALB/c mice with the DNA vaccine alone, or priming with DNA vaccine and boosting with the Loop protein, resulted in the following: (1) High-level specific antibody (IgG) against CAV-1 was induced; (2) T cell activation was elicited; and (3) neutralizing antibodies were detectable in immunized mice. Collectively, these data indicate that the availability of a DNA vaccine could prevent hepatitis contagiosa canis.

• 出版日期2007-9
• 单位中国人民解放军第二军医大学; 河北医科大学