Assessment of neuroregenerative effect of dihydrotestosterone, on peripheral nerve regeneration using allografts: a rat sciatic nerve model

作者：Karegar Mohammad; Mohammadi Rahim^*

来源：Neurological Research, 2015, 37(10): 908-915.

DOI：10.1179/1743132815Y.0000000076

• Peripheral nerve repair
• Sciatic
• Dihydrotestosterone
• Allograft
• Rat

Objectives: Effects of dihydrotestosterone on nerve allograft were studied in a rat sciatic nerve model.
Methods: 30 healthy male white Wistar rats were castrated and randomised into three experimental groups (n=10): Normal control group (NC), autograft group (AUTO), allograft group (ALLO) and dihydrotestoster-one-treated group (ALLO/DHT). In NC group, left sciatic nerve was exposed and left intact. In autograft group, a segment of sciatic nerve was transected and reimplanted reversely. In the ALLO group, the left sciatic nerve was exposed and transected where a 10-mm segment was excised. The same procedure was performed in the ALLO/DHT group. The harvested nerves of the rats of ALLO group were served as allograft for ALLO/DHT group and vice versa. The NC, AUTO and ALLO groups received 300 mu l phosphate buffered saline (PBS) intraperitoneally once a day for 1 week and the ALLO/DHT group received 300 ml DHT (1 mg/kg/day) interaperitoneally once a day for 1 week.
Results: The results showed earlier regeneration of axons in ALLO/DHT than in ALLO group (P<0.05). Histomorphometic and immunohistochemical studies also showed earlier regeneration of axons in ALLO/DHT than in ALLO group (P<0.05).
Discussions: Administration of DHT could accelerate functional recovery after nerve allografting in sciatic nerve and may have implications in clinical practice.

• 出版日期2015-10