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摘要
The hapten of alpha-S-(3-carboxy-propylcarbamoyl)-3-phenoxybenzyl-S-2-(4-Chlorophenyl)-3-methylbutyriate(Efvb) was synthesized. The haptens were conjugated with ovalbumin (OVA) as coating antigens by mixed anhydride method and with bovine serum albumin (BSA) as immunogen by active ester method. An antibody against esfenvalerate was generated by immunizing the New Zealand rabbits. After the heterologous assays and the optimization, the conditions of pH 7.4, 0.4 mol/L Na+, 40% methanol were selected as the optimum assay condition for the determination of esfenvale-rate by the indirect competitive enzyme-linked immunosorbent assay(ic-ELISA). The medium inhibitory concentration (IC50) for esfenvalerate was 3.16 +/- 0.01 mg/L, and the limit of detection (IC10) was (0.0053 +/- 0.0012) mg/L. Fenpropathrin, deltamethrin, cyhalothrin, cypermethrin and the acid of esfenvalerate metabolites have not significantly cross-reaction with antibody used in this assay. When the esfenvalerate standards were added to the tap water, river water and soil samples, the average recoveries were in the range of 82.3%-108.2%, 83.1%-109.2%, 72.0%-91.2%, respectively.
- 出版日期2012-4
- 单位南京农业大学
