Multimodal CARS microscopy of structured carbohydrate biopolymers

作者:Slepkov Aaron D*; Ridsdale Andrew; Pegoraro Adrian F; Moffatt Douglas J; Stolow Albert
来源:Biomedical Optics Express, 2010, 1(5): 1347-1357.
DOI:10.1364/BOE.1.001347

摘要

We demonstrate the utility of multimodal coherent anti-Stokes Raman scattering (CARS) microscopy for the study of structured condensed carbohydrate systems. Simultaneous second-harmonic generation (SHG) and spectrally-scanned CARS microscopy was used to elucidate structure, alignment, and density in cellulose cotton fibers and in starch grains undergoing rapid heat-moisture swelling. Our results suggest that CARS response of the O-H stretch region (3000 cm(-1)-3400 cm(-1)), together with the commonly-measured C-H stretch (2750 cm(-1)-2970 cm(-1)) and SHG provide potentially important structural information and contrast in these materials.

  • 出版日期2010-12-1